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mlm3636 guide rna expression plasmid  (Addgene inc)


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    Structured Review

    Addgene inc mlm3636 guide rna expression plasmid
    Mlm3636 Guide Rna Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mlm3636+guide+rna+expression+plasmid/MLM3636+(Plasmid+%2343860)/pmc12501846__pgaf297_supplementary_data-7-35-49
    Average 95 stars, based on 184 article reviews
    mlm3636 guide rna expression plasmid - by Bioz Stars, 2026-10
    95/100 stars

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    RNA Expression:

    Article Title: Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function
    Article Snippet: .. ESC line obtained from a male C57BL/6J mouse embryo was dissociated with TrypLE Express (Thermo Fisher Scientific), washed twice with OptiMEM (Thermo Fisher Scientific), and re-suspended in 100 μL of Opti-MEM containing 7.5 μg of MLM3636 guide RNA expression plasmid and 5 μg of Cas9 expression plasmid (MLM3636 plasmid: Addgene #43860, and pSpCas9(BB)-2A-Puro (PX459) V2.0: Addgene #62988). .. The mixture was placed in a transfection cuvette (0.2-cm gap; NEPA gene) and electroporated using a NEPA21 electroporator (NEPA Gene Co., Ltd., Chiba, Japan) with voltage, 150 V; pulse length, 5 ms; pulse interval, 50 ms; number of pulses: 2; decay rate, 10%; polarity, + for poring pulse condition; and voltage, 20 V; pulse length, 50 ms; pulse interval, 50 ms; number of pulses: 5; decay rate, 40%; polarity: +/– for transfer pulse condition.

    Plasmid Preparation:

    Article Title: Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function
    Article Snippet: .. ESC line obtained from a male C57BL/6J mouse embryo was dissociated with TrypLE Express (Thermo Fisher Scientific), washed twice with OptiMEM (Thermo Fisher Scientific), and re-suspended in 100 μL of Opti-MEM containing 7.5 μg of MLM3636 guide RNA expression plasmid and 5 μg of Cas9 expression plasmid (MLM3636 plasmid: Addgene #43860, and pSpCas9(BB)-2A-Puro (PX459) V2.0: Addgene #62988). .. The mixture was placed in a transfection cuvette (0.2-cm gap; NEPA gene) and electroporated using a NEPA21 electroporator (NEPA Gene Co., Ltd., Chiba, Japan) with voltage, 150 V; pulse length, 5 ms; pulse interval, 50 ms; number of pulses: 2; decay rate, 10%; polarity, + for poring pulse condition; and voltage, 20 V; pulse length, 50 ms; pulse interval, 50 ms; number of pulses: 5; decay rate, 40%; polarity: +/– for transfer pulse condition.

    Expressing:

    Article Title: Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function
    Article Snippet: .. ESC line obtained from a male C57BL/6J mouse embryo was dissociated with TrypLE Express (Thermo Fisher Scientific), washed twice with OptiMEM (Thermo Fisher Scientific), and re-suspended in 100 μL of Opti-MEM containing 7.5 μg of MLM3636 guide RNA expression plasmid and 5 μg of Cas9 expression plasmid (MLM3636 plasmid: Addgene #43860, and pSpCas9(BB)-2A-Puro (PX459) V2.0: Addgene #62988). .. The mixture was placed in a transfection cuvette (0.2-cm gap; NEPA gene) and electroporated using a NEPA21 electroporator (NEPA Gene Co., Ltd., Chiba, Japan) with voltage, 150 V; pulse length, 5 ms; pulse interval, 50 ms; number of pulses: 2; decay rate, 10%; polarity, + for poring pulse condition; and voltage, 20 V; pulse length, 50 ms; pulse interval, 50 ms; number of pulses: 5; decay rate, 40%; polarity: +/– for transfer pulse condition.



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    Journal: iScience

    Article Title: Cellular targets and lysine selectivity of the HERC5 ISG15 ligase

    doi: 10.1016/j.isci.2024.108820

    Figure Lengend Snippet:

    Article Snippet: MLM3636 guide RNA (gRNA) expression vector , Addgene , CAT# 43860; RRID:Addgene_43860.

    Techniques: Recombinant, Transfection, CRISPR, Peptide Fractionation, Mass Spectrometry, Expressing, Plasmid Preparation, Software